High Fidelity PCR Enzymes

Eldan offers Agilent’s variety of high fidelity PCR enzymes:

  • PfuUltra II Fusion HS DNA Polymerase 
    The industry’s standard for high-fidelity PCR, for applications such as cloning, protein crystallization studies, and sequencing. 
    PfuUltra II features a genetically engineered mutant of Pfu DNA polymerase, the ArchaeMaxx Polymerase-Enhancing Factor, fusion technology, and hot start formulation.
  • Herculase II Fusion DNA Polymerases 
    Herculase II produces superior yields with extension times as short as 15 sec/kb, even with genomic DNA targets up to 23kb. Herculase II helps overcome PCR challenges with successful amplification of targets of all complexity. It will easily amplify low abundance, high GC, and other difficult targets with robust yields.
  • PfuTurbo Cx Hotstart DNA Polymerase 
    PfuTurbo Cx formulated with a mutant of Pfu DNA polymerase that overcomes uracil stalling completely, allowing the polymerase to read through uracil located in the template strand or incorporated into the extending strand. PfuTurbo Cx has been engineered with increased proofreading activity to counter mutations introduced by uracil read-through.
  • Easy-A Hi-Fi Cloning Enzyme 
    Easy-A high-fidelity cloning enzymes combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.
  • AccuScript Hi-Fi Reverse Transcriptase
    AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

Agilent Technologies

View additional information on Agilent Genomics’ website >>


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